Phage display technology is a powerful technology based on displaying of peptide on the surface of filamentous phages established first by smith in 1985

Phage display technology is a powerful technology based on displaying of peptide on the surface of filamentous phages established first by smith in 1985. It’s a gold standard for monoclonal antibody development for various diagnostic and therapeutic aims. This technology circumvents some technical limitation in hybridoma such as repeated immunization of animals, laborious method, high cost and immunogenicity of mouse antibody for human therapeutics (15, 16). Additionally, it enables antibody production for toxic antigens as well as live cultures constrain antibody generation in animal-based antibodies production (17). For APD library preparation, most commonly applied M13 vector is utilized which needs harboring F-pilus bacteria for infection as a host. In general, antibody gene cloning occurs in fusion with gene III phage. In fact, the physical linkage between genotype and phenotype is the main concept for APD system lead to phage displaying antibody fragments. Consequently, large antibody libraries bearing of recombinant phage particles is screened on immobilized antigen, a process called bio-panning (18, 19) Then, bound phage on antigen of interest is retained an unbound ones removed. Specific binders can therefore be eluted, amplified in F positive bacterial host cells. This amplified phage pool is greatly enriched for 2-3 subsequent rounds of target affinity of selection