The study of (olive/oleic bio-alkyd resin, OOBAR) as a new biosorbent was prepared in open esterification system from an olive tree, glycerol, oleic acid and phthalic anhydride. The characterization of OOBAR was performed by using ultraviolet/visible, infrared spectra, Boehm titration, zero-point charge, iodine number, methylene blue index, density and bulk DC electrical conductivity. The data obtained show that the OOBAR surface was contained 2.6 and 1.3 mmol/g of acidic and basic sites. The pHZCP was 3.6, ?pH at pH 9.27 was -4.33, the I2 number was 2.3 mmol/g (291.9 mg/g), methylene blue was 0.69 mmol/g (220.7 mg/g), density was 0.6 g/mL and DC conductivity was 1.0 ×10-9 ?-1 cm-1. The effects of acidity, reducing agent, NH4SCN concentration, shaking time, Mo concentration, temperature, OOBAR dose, batch factor and ionic strength for the determination of Mo(V) were evaluated.